The binding between [24-(3)H]okadaic acid (OA) and a recombinant OA binding protein OABP2.1 was examined using various OA analog, including methyl okadaate, norokadanone, 7-deoxy OA, and 14,15-dihydro OA, 7-O-palmitoyl DTX1, to investigate the structure activity relationship. Among them, 7-O-palmitoyl DTX1, which is one of the diarrhetic shellfish poisoning (DSP) toxins identified in shellfish, displayed an IC50 for [24-(3)H]OA binding at 51±6.3nM (Mean±SD). In addition, a synthetic compound, N-pyrenylmethyl okadamide, exhibited its IC50 at 10±2.9nM (Mean±SD). These results suggested that the recombinant OABP2.1 and the N-pyrenylmethyl okadamide might be core substances in a novel assay for the DSP toxins.
Keywords: Diarrhetic shellfish poisoning; Dinophysistoxins; Okadaic acid; Okadaic acid binding protein.
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