Reverse transcriptase dideoxy sequencing of RNA

Abstract

During the course of studying the structure and function of RNA molecules and the proteins that they may encode, as well as studies of the expression of a given gene, there often arises a need to learn the nucleotide sequence of an RNA molecule. While the sequence of a particular RNA is ordinarily encompassed within a corresponding DNA sequence, RNA sequencing lends the advantage of giving the transcription initiation and termination sequences, and the sites where modifications from the original DNA sequence occur.

Keywords: Anneal primer; Label primer; Primer extension and chain termination; Reverse transcriptase dideoxy sequencing; UREA-PAGE.