Identification of Mycobacterium bovis Isolates by a multiplex PCR

Eduardo Eustáquio de Souza Figueiredo; Flávia Galindo Silvestre; Wilma Neres Campos; Leone Vinícius Furlanetto; Luciana Medeiros; Walter Lilenbaum; Leila Sousa Fonseca; Joab Trajano Silva; Vânia Margaret Flosi Paschoalin

doi:10.1590/S1517-83822009000200004

Identification of Mycobacterium bovis Isolates by a multiplex PCR

Braz J Microbiol. 2009 Apr;40(2):231-3. doi: 10.1590/S1517-83822009000200004. Epub 2009 Jun 1.

Authors

Eduardo Eustáquio de Souza Figueiredo¹, Flávia Galindo Silvestre, Wilma Neres Campos, Leone Vinícius Furlanetto, Luciana Medeiros, Walter Lilenbaum, Leila Sousa Fonseca, Joab Trajano Silva, Vânia Margaret Flosi Paschoalin

Affiliation

¹ Universidade Federal do Rio de Janeiro , Rio de Janeiro, RJ , Brasil ; Universidade de Cuiabá , Cuiabá, MT , Brasil.

Abstract

Isolates from suggestive bovine tuberculosis lesions were tested by a multiplex polymerase chain reaction (m-PCR) targeting for RvD1Rv2031c and IS6110 sequences, specific for M. bovis and Mycobacterium tuberculosis complex respectively. The m-PCR successfully identified as M. bovis 88.24% of the isolates.

Keywords: Mycobacterium bovis; bovine tuberculosis; multiplex-PCR.