[Cloning and sequence characterization of replication region in plasmid pJTU112 from Micoromonospora sp. 40027]

Wei Sheng Wu Xue Bao. 2013 Jun 4;53(6):623-7.
[Article in Chinese]

Abstract

Micoromonospora sp. 40027, the producer of fortimicin A, harbors two plasmids pJTU101 and pJTU112.

Objective: Cloning and sequencing of replication region of pJTU12, analyzing replication region sequence of pJTU112.

Methods: Different fragments of pJTU112 were cloned and introduced by conjugation into Micromonospora sp. LXH20. The replication region of pJTU112 was identified.

Results: The replication region of pJTU112 was located in the 4.7 kb SacI-KpnI DNA fragment. DNA sequencing and analysis revealed that the 4.7 kb SacI-KpnI DNA fragment encoded five open reading frames. The pJTU112.1 and pJTU112.2 were related to plasmid conjugation, pJTU112.3, pJTU112.4 and pJTU112.5 were related to plasmid conjugation.

Conclusion: The replication region of pJTU112 was located in the 4.7kb SacI-KpnI DNA fragment.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Cloning, Molecular*
  • Micromonospora / genetics*
  • Open Reading Frames
  • Plasmids / genetics*
  • Replication Origin*
  • Sequence Analysis, DNA

Substances

  • Bacterial Proteins