Micoromonospora sp. 40027, the producer of fortimicin A, harbors two plasmids pJTU101 and pJTU112.
Objective: Cloning and sequencing of replication region of pJTU12, analyzing replication region sequence of pJTU112.
Methods: Different fragments of pJTU112 were cloned and introduced by conjugation into Micromonospora sp. LXH20. The replication region of pJTU112 was identified.
Results: The replication region of pJTU112 was located in the 4.7 kb SacI-KpnI DNA fragment. DNA sequencing and analysis revealed that the 4.7 kb SacI-KpnI DNA fragment encoded five open reading frames. The pJTU112.1 and pJTU112.2 were related to plasmid conjugation, pJTU112.3, pJTU112.4 and pJTU112.5 were related to plasmid conjugation.
Conclusion: The replication region of pJTU112 was located in the 4.7kb SacI-KpnI DNA fragment.