Chemically induced pluripotent stem cells (CiPSCs): a transgene-free approach

Shigeo Masuda; Jun Wu; Tomoaki Hishida; Ganesh N Pandian; Hiroshi Sugiyama; Juan Carlos Izpisua Belmonte

doi:10.1093/jmcb/mjt034

Chemically induced pluripotent stem cells (CiPSCs): a transgene-free approach

J Mol Cell Biol. 2013 Oct;5(5):354-5. doi: 10.1093/jmcb/mjt034. Epub 2013 Sep 6.

Authors

Shigeo Masuda¹, Jun Wu, Tomoaki Hishida, Ganesh N Pandian, Hiroshi Sugiyama, Juan Carlos Izpisua Belmonte

Affiliation

¹ Gene Expression Laboratory, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA.

PMID: 24014886
DOI: 10.1093/jmcb/mjt034

Abstract

Induced pluripotent stem cells (iPSCs) could be generated by a single gene Oct4 and chemical compounds, in which exogenous expression of Oct4 was indispensable for reprogramming. Recent advances in chemical-mediated cellular reprogramming suggest that small molecules alone (i.e. without Yamanaka factors) can successfully establish iPSCs from mouse somatic cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cellular Reprogramming / drug effects
Colforsin / pharmacology*
Embryo, Mammalian / cytology
Fibroblasts / cytology
Fibroblasts / drug effects
Fibroblasts / metabolism
Green Fluorescent Proteins / metabolism
Humans
Induced Pluripotent Stem Cells / cytology*
Induced Pluripotent Stem Cells / drug effects
Induced Pluripotent Stem Cells / metabolism*
Mice
Octamer Transcription Factor-3 / metabolism
Transgenes / genetics*

Substances

Octamer Transcription Factor-3
Green Fluorescent Proteins
Colforsin