Characterisation of CMP-sialic acid transporter substrate recognition

Chembiochem. 2013 Oct 11;14(15):1936-42. doi: 10.1002/cbic.201300298. Epub 2013 Sep 6.

Abstract

CMP-sialic acid transporter: We report an in-depth, multidisciplinary, structural study that has identified the amino acid residues intimately involved in CMP-sialic acid transporter (CST) substrate specificity. Our data provide a significant contribution towards a better understanding the structure-function relationship of this important family of transporters and the rational design of CST inhibitors.

Keywords: CMP-sialic acid transporter; NMR spectroscopy; nucleotide sugar transporters; sialic acids; site-directed mutagenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Cell Line
  • Cell Membrane / metabolism
  • Cytidine Monophosphate / metabolism*
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed
  • Mutation
  • Organic Anion Transporters / chemistry
  • Organic Anion Transporters / genetics
  • Organic Anion Transporters / metabolism*
  • Protein Binding
  • Protein Structure, Tertiary
  • Substrate Specificity
  • Symporters / chemistry
  • Symporters / genetics
  • Symporters / metabolism*

Substances

  • Organic Anion Transporters
  • Symporters
  • sialic acid transport proteins
  • Cytidine Monophosphate