The use of an engineered single chain variable fragment in a localized surface plasmon resonance method for analysis of the C-reactive protein

Chem Commun (Camb). 2013 Oct 21;49(82):9497-9. doi: 10.1039/c3cc45046e.

Abstract

A gold nanorod (GNR) based LSPR sensor has been developed for label-free detection of C-reactive protein (CRP). The sensor utilizes a single chain variable fragment (scFv) as a receptor to bind CRP. The results of this effort show that CRP in human serum can be quantitatively detected at lower than 1 ng mL(-1).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blood Chemical Analysis / methods*
  • C-Reactive Protein / chemistry*
  • C-Reactive Protein / metabolism
  • Gold / chemistry
  • Humans
  • Limit of Detection
  • Nanotubes / chemistry
  • Single-Chain Antibodies / chemistry*
  • Single-Chain Antibodies / metabolism
  • Surface Plasmon Resonance*

Substances

  • Single-Chain Antibodies
  • Gold
  • C-Reactive Protein