Explanatory chapter: troubleshooting PCR

Methods Enzymol. 2013:529:271-8. doi: 10.1016/B978-0-12-418687-3.00022-7.

Abstract

The purpose of this chapter is to assist with any problems you are having with the polymerase chain reaction (PCR) protocol from General PCR.

Keywords: Annealing; Band amplification; DNA template; Denaturation; Extension; Taq DNA polymerase and primers; Troubleshooting PCR.

MeSH terms

  • DNA Primers
  • Polymerase Chain Reaction / methods*
  • Taq Polymerase / chemistry*

Substances

  • DNA Primers
  • Taq Polymerase