Sanger dideoxy sequencing of DNA

Abstract

While the ease and reduced cost of automated DNA sequencing has largely obviated the need for manual dideoxy sequencing for routine purposes, specific applications require manual DNA sequencing. For instance, in studies of enzymes or proteins that bind or modify DNA, a DNA ladder is often used to map the site at which an enzyme is bound or a modification occurs. In these cases, the Sanger method for dideoxy sequencing provides a rapid and facile method for producing a labeled DNA ladder.

Keywords: DNA labeling; Dideoxy sequencing of DNA; Primer annealing; Sanger method; Urea-PAGE.