The anti-inflammatory property of human bone marrow-derived mesenchymal stem/stromal cells is preserved in late-passage cultures

J Neuroimmunol. 2013 Oct 15;263(1-2):55-63. doi: 10.1016/j.jneuroim.2013.07.018. Epub 2013 Aug 6.

Abstract

Human mesenchymal stem/stromal cells (hMSCs) have been reported to improve neural damage via anti-inflammation and multi-differentiation abilities. Here, we investigated immunosuppression effects of hMSCs by mixed-culturing with interferon-γ (IFNγ) stimulated BV-2 mouse microglial cells. We show that hMSCs decreased nitrite oxide (NO) production from BV-2 cells in cell density dependent manner. Aged hMSCs and peroxisome proliferator-activated receptor-γ (PPARγ) knockdown hMSCs decreased differentiation abilities but maintained NO suppressive function. We finally confirmed NO suppression activities of hMSCs in IFNγ-stimulated primary microglia/macrophages. It suggested that hMSCs significantly modified NO production in activated phagocytes and it might be preserved in late passage cultures.

Keywords: Human mesenchymal stem/stromal cells; Inflammation; Interferon-γ; Microglial cells; Nitric oxide.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone Marrow Cells / immunology*
  • Bone Marrow Cells / pathology
  • Cell Culture Techniques / methods
  • Cells, Cultured
  • Coculture Techniques
  • Humans
  • Inflammation / immunology
  • Inflammation / pathology
  • Inflammation / prevention & control
  • Inflammation Mediators / antagonists & inhibitors*
  • Inflammation Mediators / physiology
  • Mesenchymal Stem Cells / immunology*
  • Mesenchymal Stem Cells / pathology
  • Mice
  • Mice, Inbred C57BL
  • Microglia / immunology
  • Microglia / pathology
  • Nitric Oxide / antagonists & inhibitors
  • Nitric Oxide / immunology

Substances

  • Inflammation Mediators
  • Nitric Oxide