Application of an ELISA-type screen printed electrode-based potentiometric assay to the detection of Cryptosporidium parvum oocysts

Olivier Laczka; Lucy Skillman; William G Ditcham; Brenton Hamdorf; Danny K Y Wong; Peter Bergquist; Anwar Sunna

doi:10.1016/j.mimet.2013.08.014

Application of an ELISA-type screen printed electrode-based potentiometric assay to the detection of Cryptosporidium parvum oocysts

J Microbiol Methods. 2013 Nov;95(2):182-5. doi: 10.1016/j.mimet.2013.08.014. Epub 2013 Aug 29.

Authors

Olivier Laczka¹, Lucy Skillman, William G Ditcham, Brenton Hamdorf, Danny K Y Wong, Peter Bergquist, Anwar Sunna

Affiliation

¹ Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW 2109, Australia; Environmental Biotechnology CRC, Australian Technology Park, Eveleigh, NSW 2006, Australia. Electronic address: Olivier.Laczka@uts.edu.au.

PMID: 23994166
DOI: 10.1016/j.mimet.2013.08.014

Abstract

We report a novel electrochemical method for the rapid detection of the parasitic protozoan, Cryptosporidium parvum. An antibody-based capture format was transferred onto screen-printed electrodes and the presence of horseradish peroxidase-labelled antibodies binding to the oocysts was potentiometrically detected. This method allowed the detection of 5 × 10(2)Cryptosporidium oocysts per mL in 60 min.

Keywords: Antibodies; Cryptosporidium oocysts; Potentiometry; Screen-printed electrodes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antibodies / chemistry
Cryptosporidium parvum / genetics
Cryptosporidium parvum / isolation & purification*
Electrodes
Enzyme-Linked Immunosorbent Assay / methods*
Oocysts*
Potentiometry / methods*

Substances

Antibodies