Seven partial-length cDNAs and 1 full-length cDNA that were involved in carotenoid biosynthesis and 2 partial-length cDNAs that encoded carotenoid cleavage dioxygenases were first isolated and characterised in 2 tartary buckwheat cultivars (Fagopyrum tataricum Gaertn.), Hokkai T8 and Hokkai T10. They were constitutively expressed at high levels in the leaves and flowers, where carotenoids are mostly distributed. During the seed development of tartary buckwheat, an inverse correlation between transcription level of carotenoid cleavage dioxygenase and carotenoid content was observed. The light-grown sprouts exhibited higher levels of expression of carotenoid biosynthetic genes in T10 and carotenoid content in both T8 and T10 compared to the dark-grown sprouts. The predominant carotenoids in tartary buckwheat were lutein and β-carotene, and very abundant amounts of these carotenoids were found in light-grown sprouts. This study might broaden our understanding of the molecular mechanisms involved in carotenoid biosynthesis and indicates targets for increasing the production of carotenoids in tartary buckwheat.
Keywords: 9-cis-epoxycarotenoid dioxygenase; ABA; CCD; CHXB; CHXE; Carotenoids; DAS; DEPC; GGDP; Gene characterization; HPLC; LCYB; LCYE; NCED; PDS; PSY; Seed; Sprout; Tartary buckwheat; ZDS; ZEP; abscisic acid; carotenoid cleavage dioxygenase; days after sowing; diethylpyrocarbonate; geranylgeranyl diphosphate; high-performance liquid chromatography; lycopene β-cyclase; lycopene ε-cyclase; phytoene desaturase; phytoene synthase; zeaxanthin epoxidase; β-ring carotene hydroxylase; ε-ring carotene hydroxylase; ξ-carotene desaturase.
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