Abstract
The enrichment of HIV-1 macromolecules at the uropod of polarized T cells can significantly promote virus assembly and cell-mediated infection. Using live-cell fluorescence microscopy, we demonstrate that full-length HIV-1 RNA is enriched at the uropod membrane; furthermore, the presence of HIV-1 Gag containing a functional nucleocapsid domain is necessary for this HIV-1 RNA enrichment. The results from these studies provide novel insights into the mechanism of HIV-1 replication in polarized T cells.
Publication types
-
Research Support, N.I.H., Extramural
-
Research Support, N.I.H., Intramural
-
Research Support, Non-U.S. Gov't
MeSH terms
-
CD4-Positive T-Lymphocytes / virology*
-
Cell Line
-
Cell Membrane / virology*
-
HIV-1 / physiology*
-
Humans
-
Macromolecular Substances / metabolism*
-
Microscopy, Fluorescence
-
RNA, Viral / metabolism*
-
Virus Assembly*
-
gag Gene Products, Human Immunodeficiency Virus / metabolism*
Substances
-
Macromolecular Substances
-
RNA, Viral
-
gag Gene Products, Human Immunodeficiency Virus