Effect of heat-shock induced oxidative stress is suppressed in BcZAT12 expressing drought tolerant tomato

Abstract

The transcription factor ZAT12 is a member of stress-responsive C2H2 type zinc finger protein (ZFP) reported to control the expression of stress-activated genes mediated via ROS in plants. BcZAT12-transformed tomato cv. H-86, var. Kashi vishesh (lines ZT1-ZT6) over-expressing the gene product is demonstrated herein to be tolerant to heat-shock (HS)-induced oxidative stress. Results reveal that the relative expression of ZAT12 as well as heat induced Hsp17.4 and Hsp21 gene transcripts increased in transgenic upon exposure to HS. The transformed tomato lines ZT1 and ZT5 had significantly lowered free radical formation, improved electrolyte leakage, relative water content and chlorophyll levels with an enhanced activities of antioxidant enzymes viz. superoxide dismutase, catalase, ascorbate peroxidase and glutathione reductase when exposed to HS. HS-induced oxidative stress by over-expression of the BcZAT12 gene transcripts in tomato as well as by largely enhancing the ROS-scavenging capacity and up regulation of Hsp transcripts. This enables the transgenic tomato plants to acquire a greater ability to counteract HS-induced oxidative stress, being endowed with more reduced antioxidant pools. The use of these HS-tolerant tomato lines could possibly be used for tomato cultivation in the areas affected by sudden temperature changes.

Keywords: APX; Antioxidant; CAT; CCI; EL; Free radicals; GR; HS; Hsp; NT; ROS; RWC; SOD; T; Tomato; ZAT12; ZFP; Zinc finger protein; ascorbate peroxidase; catalase; chlorophyll color index; electrolyte leakage; glutathione reductase; heat shock protein; heat stress; non-transformed; reactive oxygen species; relative water content; superoxide dismutase; transformed; zinc finger protein.