A headspace gas chromatographic method (HS-GC) has been developed for the determination of biological oxygen demand (BOD) in the water samples from domestic wastewater treatment plants. The method is based on measuring the remaining oxygen in the headspace of the sample (that has been seeded with microorganisms) in a closed container after a period of incubation. The relative standard deviation of the method in replicate testing was <4.9%. Further, the results differed by less than 6% when compared with the widely used reference method (the ISO standard) for determining BOD5. The limit of quantification in BODn testing was about 1.8 mg/L. The new method is simple and suitable for use in the batch sample testing for BODn measurement.
Keywords: BOD; Dissolved oxygen; Domestic wastewater; GC; Headspace.
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