Abstract
Phalloidin, an actin-filament stabilizing peptide from Amanita phalloides, did not inhibit ATP hydrolysis during actin polymerization but strongly retarded the release of the hydrolysis product Pi. Thus, the lifetime of the intermediate F-actin-ADP-Pi is significantly increased by phalloidin. The results suggest a close correlation between filament stability and F-actin-ADP-Pi intermediates.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Actins / isolation & purification
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Actins / metabolism*
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Adenosine Triphosphate / metabolism
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Animals
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Kinetics
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Macromolecular Substances
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Muscles / metabolism
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Oligopeptides / pharmacology*
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Phalloidine / pharmacology*
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Phosphates / metabolism*
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Rabbits
Substances
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Actins
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Macromolecular Substances
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Oligopeptides
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Phosphates
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Phalloidine
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Adenosine Triphosphate