Ultra high performance liquid chromatography with ion-trap TOF-MS for the fast characterization of flavonoids in Citrus bergamia juice

J Sep Sci. 2013 Oct;36(20):3351-5. doi: 10.1002/jssc.201300591. Epub 2013 Aug 28.

Abstract

We have developed a fast ultra HPLC with ion-trap TOF-MS method for the analysis of flavonoids in Citrus bergamia juice. With respect to the typical methods for the analysis of these matrices based on conventional HPLC techniques, a tenfold faster separation was attained. The use of a core-shell particle column ensured high resolution within the fast analysis time of only 5 min. Unambiguous determination of flavonoid identity was obtained by the employment of a hybrid ion-trap TOF mass spectrometer with high mass accuracy (average error 1.69 ppm). The system showed good retention time and peak area repeatability, with maximum RSD% values of 0.36 and 3.86, respectively, as well as good linearity (R(2) ≥ 0.99). Our results show that ultra HPLC can be a useful tool for ultra fast qualitative/quantitative analysis of flavonoid compounds in citrus fruit juices.

Keywords: Bergamot; Citrus; Core-Shell; Flavonoids; Mass accuracy.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Beverages / analysis*
  • Chromatography, High Pressure Liquid / methods*
  • Citrus / chemistry*
  • Flavonoids / chemistry*
  • Fruit / chemistry
  • Mass Spectrometry / methods*
  • Molecular Structure
  • Plant Extracts / chemistry*

Substances

  • Flavonoids
  • Plant Extracts