[Analysis of full-length gene sequence of rabies vaccine virus aG strain]

Bing Du Xue Bao. 2013 Jun;29(4):404-9.
[Article in Chinese]

Abstract

To sequence and analyze the full-length gene sequence of rabies vaccine virus aG strain. The full-length gene sequence of aG strain was amplified by RT-PCR by 8 fragments,each PCR product was cloned into vector pGEM-T respectively, sequenced and assemblied; The 5' leader sequence was sequenced with method of 5' RACE. The homology between aG and other rabies vaccine virus was analyzed by using DNAstar and Mega4. 0 software. aG strain was 11 925nt(GenBank accession number: JN234411) in length and belonged to the genotype I . The Bioinformatics revealed that the homology showed disparation form different rabies vaccine virus. the full-length gene sequence of rabies vaccine virus aG strain provided a support for perfecting the standard for quality control of virus strains for production of rabies vaccine for human use in China.

Publication types

  • English Abstract

MeSH terms

  • Amino Acid Sequence
  • Antigens, Viral / genetics
  • Antigens, Viral / immunology*
  • Base Sequence
  • China
  • Genome, Viral / genetics*
  • Genotype
  • Humans
  • Molecular Sequence Data
  • Phylogeny
  • Rabies / immunology
  • Rabies / prevention & control
  • Rabies / virology*
  • Rabies Vaccines / immunology*
  • Rabies virus / genetics*
  • Rabies virus / immunology
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Species Specificity

Substances

  • Antigens, Viral
  • Rabies Vaccines

Associated data

  • GENBANK/JN234411