The Crosslink formation of 2'-OMe oligonucleotide containing 2-amino-6-vinylpurine protects mRNA from miRNA-mediated silencing

Chembiochem. 2013 Aug 19;14(12):1427-9. doi: 10.1002/cbic.201300382. Epub 2013 Jul 24.

Abstract

Masking the miRNA binding site: Crosslink-forming oligonucleotide (CFO) was used for target gene-specific inhibition of microRNA (miRNA) functions. This method can interfere with specific miRNA-mRNA interactions by recognizing sequences unique to the 3'-UTR that are inherent in each mRNA.

Keywords: crosslinks; gene expression; mRNA; miRNA; oligonucleotides.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Binding Sites / drug effects
  • Cross-Linking Reagents / chemistry
  • Cross-Linking Reagents / pharmacology*
  • Fireflies
  • Gene Silencing / drug effects*
  • HeLa Cells
  • Humans
  • Luciferases / chemistry
  • Luciferases / genetics
  • MicroRNAs*
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides / chemistry
  • Oligodeoxyribonucleotides / pharmacology*
  • Purines*
  • RNA, Messenger / chemistry*
  • RNA, Messenger / metabolism
  • Vinyl Compounds*

Substances

  • 2-amino-6-vinylpurine
  • Cross-Linking Reagents
  • MicroRNAs
  • Oligodeoxyribonucleotides
  • Purines
  • RNA, Messenger
  • Vinyl Compounds
  • Luciferases