A suite of solid-state NMR experiments for RNA intranucleotide resonance assignment in a 21 kDa protein-RNA complex

Alexander Marchanka; Bernd Simon; Teresa Carlomagno

doi:10.1002/anie.201304779

A suite of solid-state NMR experiments for RNA intranucleotide resonance assignment in a 21 kDa protein-RNA complex

Angew Chem Int Ed Engl. 2013 Sep 16;52(38):9996-10001. doi: 10.1002/anie.201304779. Epub 2013 Jul 26.

Authors

Alexander Marchanka¹, Bernd Simon, Teresa Carlomagno

Affiliation

¹ Structural and Computational Biology Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstr. 1, 69117 Heidelberg (Germany).

PMID: 23893717
DOI: 10.1002/anie.201304779

Abstract

Intranucleotide resonance of the 26mer box C/D RNA in complex with the L7Ae protein were assigned by solid-state NMR (ssNMR; see picture) spectroscopy. This investigation opens the way for studying RNA in large protein-RNA complexes by ssNMR spectroscopy.

Keywords: NMR spectroscopy; RNA; RNA-protein complexes; structure elucidation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Magnetic Resonance Spectroscopy / methods*
Molecular Structure
Proteins / chemistry*
RNA / chemistry*

Substances

Proteins
RNA