The lectin of Dolichos biflorus agglutinin recognises glycan epitopes on the surface of a subset of cardiac progenitor cells

Cell Biol Int. 2013 Nov;37(11):1238-45. doi: 10.1002/cbin.10162. Epub 2013 Aug 13.

Abstract

The discovery of adult cardiac progenitor cells (CPCs) provides a promising way for treating heart disease; however, their surface characteristics that play a critical role in regulating their maintenance, self-renewal, migration, and differentiation have not been fully investigated. One subpopulation of Dolichos biflorus agglutinin (DBA)-positive cells was identified in the heart of adult mice. Flow cytometry showed that 3.7% of heart cells could be labeled by FITC conjugated DBA. BrdU pulse-chase showed that 55-75% of DBA(+) cells were CPCs. Evidences from 5-FU-induced myelosuppression along with BrdU pulse-chasing suggests that DBA-positive cells are proliferative. Furthermore, DBA positive cells display a cologenic appearance in vivo. Our findings suggest that DBA-positive cells in the heart of adult mouse contained a subset of CPCs, and DBA reactivity is one novel surface characteristic on CPCs.

Keywords: BrdU pulse chasing; Dolichos biflorus agglutinin; cardiac progenitor cells; niche.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bromodeoxyuridine / metabolism
  • Cell Proliferation / drug effects
  • Clone Cells
  • Epitopes / immunology*
  • Fluorouracil / pharmacology
  • Mice
  • Mice, Inbred ICR
  • Myocardium / cytology*
  • Plant Lectins / immunology*
  • Polysaccharides / immunology*
  • Proto-Oncogene Proteins c-kit / metabolism
  • Staining and Labeling
  • Stem Cells / cytology*
  • Stem Cells / drug effects
  • Stem Cells / immunology*

Substances

  • Epitopes
  • Plant Lectins
  • Polysaccharides
  • dolichos biflorus agglutinin
  • Proto-Oncogene Proteins c-kit
  • Bromodeoxyuridine
  • Fluorouracil