Eight Polybrominated diphenyl ether (PBDE) congeners (BDE 28, 47, 99, 100, 153, 154, 183 and 209) were determined to examine the hair burden at low concentrations, and the relationship between PBDE concentrations in human hair and indoor dust from a college environment (Shanghai University campus). Chemical analyses showed that the total concentrations of PBDEs in hair ranged from 4.04 to 99 ng/g dw, and were found to be fourfold higher in females than in males (p < 0.05). The total PBDEs concentrations in indoor dust samples ranged from 170 to 1,360 ng/g dw. Significantly positive correlations were observed between human hair and indoor dust for BDE 47 (r = 0.44, p = 0.048) and BDE 99 (r = 0.68, p = 0.025). However, no significant association was noted between other PBDE congeners in human hair and indoor dust in the present study.