Ethanol fermentation from xylose by metabolically engineered strains of Kluyveromyces marxianus

Tetsuya Goshima; Kanako Negi; Masaharu Tsuji; Hiroyuki Inoue; Shinichi Yano; Tamotsu Hoshino; Akinori Matsushika

doi:10.1016/j.jbiosc.2013.05.010

Ethanol fermentation from xylose by metabolically engineered strains of Kluyveromyces marxianus

J Biosci Bioeng. 2013 Nov;116(5):551-4. doi: 10.1016/j.jbiosc.2013.05.010. Epub 2013 Jul 18.

Authors

Tetsuya Goshima¹, Kanako Negi, Masaharu Tsuji, Hiroyuki Inoue, Shinichi Yano, Tamotsu Hoshino, Akinori Matsushika

Affiliation

¹ Biomass Refinery Research Center (BRRC), National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashi-hiroshima, Hiroshima 739-0046, Japan.

PMID: 23871200
DOI: 10.1016/j.jbiosc.2013.05.010

Abstract

We constructed a xylose-fermenting recombinant strain of thermotolerant yeast Kluyveromyces marxianus, DMB3-7. Both xylose consumption and ethanol production were remarkably increased in DMB3-7 compared to the control strain at 30°C. Furthermore, DMB3-7 produced ethanol from xylose at both 42°C and 45°C, above which xylose metabolic activity decreased.

Keywords: Bioethanol; Thermotolerant yeast; Xylitol dehydrogenase; Xylose; Xylose reductase; Xylulokinase.

MeSH terms

Aldehyde Reductase / metabolism
Biofuels / supply & distribution
D-Xylulose Reductase / metabolism
Ethanol / metabolism*
Fermentation*
Kluyveromyces / genetics*
Kluyveromyces / metabolism*
Metabolic Engineering*
Phosphotransferases (Alcohol Group Acceptor) / metabolism
Temperature
Xylose / metabolism*

Substances

Biofuels
Ethanol
Xylose
Aldehyde Reductase
D-Xylulose Reductase
Phosphotransferases (Alcohol Group Acceptor)
xylulokinase