In this article, the expression patterns of LRF in the mouse oviduct, uterus, and ovary were checked during estrous cycle. The expression of LRF mRNA and protein were remarkably changed in the ovary, oviduct, and uterus at four phases. LRF immunostaining was detected in the follicle from primary to antral follicle, luminal and glandular epithelial cells of the uterus, and cilia of the ciliated cells of the oviduct at all phase. Our findings suggested that LRF may be related to the processes of development and maturation of oocyte, gamete transport, and the development of early embryo.