Investigation of macrophage polarization using bone marrow derived macrophages

Wei Ying; Patali S Cheruku; Fuller W Bazer; Stephen H Safe; Beiyan Zhou

doi:10.3791/50323

Investigation of macrophage polarization using bone marrow derived macrophages

J Vis Exp. 2013 Jun 23:(76):50323. doi: 10.3791/50323.

Authors

Wei Ying¹, Patali S Cheruku, Fuller W Bazer, Stephen H Safe, Beiyan Zhou

Affiliation

¹ Department of Animal Science, Texas A&M University, TX, USA.

PMID: 23851980
PMCID: PMC3728835
DOI: 10.3791/50323

Abstract

The article describes a readily easy adaptive in vitro model to investigate macrophage polarization. In the presence of GM-CSF/M-CSF, hematopoietic stem/progenitor cells from the bone marrow are directed into monocytic differentiation, followed by M1 or M2 stimulation. The activation status can be tracked by changes in cell surface antigens, gene expression and cell signaling pathways.

Publication types

Research Support, Non-U.S. Gov't
Video-Audio Media

MeSH terms

Animals
Bone Marrow Cells / cytology
Bone Marrow Cells / drug effects
Cell Differentiation / drug effects
Cell Polarity / drug effects
Cell Polarity / physiology
Cytological Techniques / methods*
Granulocyte-Macrophage Colony-Stimulating Factor / pharmacology
Hematopoietic Stem Cells / cytology
Hematopoietic Stem Cells / drug effects
Macrophage Activation / drug effects
Macrophage Activation / physiology
Macrophage Colony-Stimulating Factor / pharmacology
Macrophages / cytology*
Macrophages / drug effects
Mice

Substances

Macrophage Colony-Stimulating Factor
Granulocyte-Macrophage Colony-Stimulating Factor