[Research of DNA barcode of Teseudinis Carapax et Planstrum and its adulterants based on COI gene sequence]

Zhongguo Zhong Yao Za Zhi. 2013 Apr;38(7):947-50.
[Article in Chinese]

Abstract

Objective: To use COI gene on the Mauremys reevesii and its adulterants by molecular identification. Search a rapid, accurate method of identification of Teseudinis Carapax et Planstrum and its adulterants.

Method: We collected 8 species of the authentic and adulterants of teseudinis carapax et planstrum in a nationwide then, extracted DNA, got the COI sequences. Use ContigExpress, Dnaman, Edit Sequence and Mega 5 to analyze the variable site and construct the N-J tree.

Result: Compare with the authentic Teseudinis Carapax et Planstrum, the adulterant exist lots of variable site. The N-J tree Indicates that the same genus belong together and each species belong to relatively independent branch.

Conclusion: Based on the COI gene, the technology of DNA bar code can be a excellent identification of Teseudinis Carapax et Planstrum and its adulterants.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • DNA Barcoding, Taxonomic
  • Electron Transport Complex IV / genetics*
  • Medicine, Chinese Traditional / standards*
  • Molecular Sequence Data
  • Phylogeny
  • Quality Control
  • Reptilian Proteins / genetics*
  • Sequence Analysis, DNA
  • Turtles / classification*
  • Turtles / genetics*

Substances

  • Reptilian Proteins
  • Electron Transport Complex IV