A new cytotoxic carbazole alkaloid and two new other alkaloids from Clausena excavata

Chem Biodivers. 2013 Jul;10(7):1317-21. doi: 10.1002/cbdv.201200395.

Abstract

One new carbazole alkaloid, excavatine A (1), and two additional new alkaloids, excavatine B (2) and excavatine C (3), were isolated from the stems and leaves of Clausena excavata Burm.f. (Rutaceae). Their structures were determined on the basis of detailed spectroscopic analyses, especially 2D-NMR and HR-EI-MS data. Compounds 1-3 were tested for their cytotoxic activities against A549, HeLa, and BGC-823 cancer cell lines, and for their antimicrobial activities against Candida albicans and Staphylococcus aureus. Only 1 exhibited cytotoxicity against A549 and HeLa cell lines with the IC50 values of 5.25 and 1.91 μg/ml, respectively.

Keywords: Alkaloids; Carbazole alkaloids; Clausena excavata; Cytotoxic activity; Excavatines A - C.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkaloids / chemistry*
  • Alkaloids / isolation & purification
  • Alkaloids / toxicity
  • Anti-Infective Agents / chemistry*
  • Anti-Infective Agents / isolation & purification
  • Anti-Infective Agents / toxicity*
  • Candida albicans / drug effects
  • Carbazoles / chemistry*
  • Carbazoles / isolation & purification
  • Carbazoles / toxicity
  • Cell Line, Tumor
  • Cell Survival / drug effects
  • Clausena / chemistry*
  • HeLa Cells
  • Humans
  • Magnetic Resonance Spectroscopy
  • Molecular Conformation
  • Plant Leaves / chemistry
  • Plant Stems / chemistry
  • Staphylococcus aureus / drug effects

Substances

  • Alkaloids
  • Anti-Infective Agents
  • Carbazoles
  • excavatine A
  • carbazole