Two new steroids from an endophytic fungus Phomopsis sp

Chem Biodivers. 2013 Jul;10(7):1276-83. doi: 10.1002/cbdv.201200415.

Abstract

Two new steroids, (14β,22E)-9,14-dihydroxyergosta-4,7,22-triene-3,6-dione (1) and (5α,6β,15β,22E)-6-ethoxy-5,15-dihydroxyergosta-7,22-dien-3-one (2), together with three known steroids, calvasterols A and B (3 and 4, resp.), and ganodermaside D (5), were isolated from the culture broth of an endophytic fungus Phomopsis sp. isolated from Aconitum carmichaeli. The structures of these compounds were elucidated on the basis of spectroscopic analysis, and their inhibitory activities against six pathogenic fungi were evaluated. Most of the compounds showed moderate or weak antifungal activities in a broth-microdilution assay.

Keywords: Antifungal activity; Fungi; Inhibitors; Phomopsis; Steroids.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antifungal Agents / chemistry*
  • Antifungal Agents / isolation & purification
  • Antifungal Agents / pharmacology
  • Ergosterol / analogs & derivatives*
  • Ergosterol / chemistry
  • Ergosterol / isolation & purification
  • Ergosterol / pharmacology
  • Fungi / drug effects
  • Magnetic Resonance Spectroscopy
  • Molecular Conformation
  • Saccharomycetales / chemistry
  • Saccharomycetales / metabolism*
  • Steroids / chemistry*
  • Steroids / isolation & purification
  • Steroids / pharmacology

Substances

  • 6-ethoxy-5,15-dihydroxyergosta-7,22-dien-3-one
  • 9,14-dihydroxyergosta-4,7,22-triene-3,6-dione
  • Antifungal Agents
  • Steroids
  • Ergosterol