CO2 bioconversion using carbonic anhydrase (CA): effects of PEG rigidity on the structure of bio-mineralized crystal composites

J Biotechnol. 2013 Oct 20;168(2):208-11. doi: 10.1016/j.jbiotec.2013.06.024. Epub 2013 Jul 9.

Abstract

The combined effect of both carbonic anhydrase (CA) and the rigidity of polyethylene glycol (PEG) were found to assist the bio-mineralized crystallization behavior of CO2 differentially. In this study, different forms of magnetically responsive calcium carbonate (CaCO3) crystal composites were successfully formed from gaseous CO2 by using the different forms of polyethylene glycols (PEGs) in a constant CO2 pressure controlled chamber. Polygonal particles were produced with more rigid polymer chains (branched PEG), whereas less rigid polymer chains (PEG) induced the formation of ellipsoidal particles. However, no morphological changes occurred without the presence of CA.

Keywords: Biomineralization; Calcium carbonate; Carbon dioxide; Carbonic anhydrase; Polymer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Calcium Carbonate / chemistry*
  • Calcium Carbonate / metabolism*
  • Carbon Dioxide / metabolism*
  • Carbonic Anhydrases / metabolism*
  • Crystallization / methods
  • Environment, Controlled
  • Polyethylene Glycols / chemistry*
  • Polymers / chemistry*
  • Pressure

Substances

  • Polymers
  • Carbon Dioxide
  • Polyethylene Glycols
  • Carbonic Anhydrases
  • Calcium Carbonate