Abstract
The ability of short peptide nucleic acid (PNA) oligomers and oligonucleotides containing modified residues of 5-methylcitidine, 2-aminoadenosine and 5-propynyl-2'-deoxyuridine (strong binding oligonucleotides, SBO) to affinity capture the target double-stranded DNA fragment from mixture by means of the end invasion was compared. Both types of probes were highly effective at the conditions used. The SBO-based probes may represent a handy and easily prepared alternative to PNA for selection of target DNA fragments from mixtures.
MeSH terms
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Adenosine / analogs & derivatives
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Adenosine / chemistry
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Base Sequence
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DNA / chemistry*
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DNA / genetics
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DNA / isolation & purification
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Humans
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Oligonucleotides / chemical synthesis*
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Oligonucleotides / chemistry
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Peptide Nucleic Acids / chemical synthesis*
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Peptide Nucleic Acids / chemistry
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Peptide Nucleic Acids / genetics
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Polymerase Chain Reaction
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Promoter Regions, Genetic
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Repressor Proteins / chemistry
Substances
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Oligonucleotides
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Peptide Nucleic Acids
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Repressor Proteins
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ZBTB32 protein, human
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2-aminoadenosine
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DNA
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Adenosine