miR-210 regulates the interaction between pancreatic cancer cells and stellate cells

Abstract

There is accumulating evidence that pancreatic stellate cells (PSCs) promote the progression of pancreatic cancer. microRNAs (miRNAs) are small non-coding RNAs acting as negative regulators of gene expression at the post-transcriptional level. This study aimed to clarify the role of miRNAs in the interaction between PSCs and pancreatic cancer cells. Pancreatic cancer cells were mono-cultured or indirectly co-cultured with PSCs. miRNAs were prepared, and Agilent's miRNA microarray containing probes for 904 human miRNAs was used to identify differentially expressed miRNAs. miR-210 was identified as an upregulated miRNA by co-culture with PSCs. Conditioned media of PSCs activated ERK and Akt, but not hypoxia-inducible factor-1α pathway. PSCs-induced miR-210 upregulation was inhibited by inhibitors of ERK and PI3K/Akt pathways. Inhibition of miR-210 expression decreased migration, decreased the expression of vimentin and snai-1, and increased the membrane-associated expression of β-catenin in Panc-1 cells co-cultured with PSCs. In conclusion, our results suggest a novel role of miR-210 in the interaction between PSCs and pancreatic cancer cells.

Keywords: 4′, 6-diamidino-2-phenylinodole; 5-bromo-2′-deoxyuridine; BrdU; DAPI; EMT; Fibrosis; HIF; MicroRNA; OD; PSC-CM; PSCs; Pancreatic cancer; Pancreatitis; Stroma; conditioned medium of hPSC21-S/T cells; epithelial-mesenchymal transition; hypoxia-inducible factor; miRNA; micro RNA; optical density; pancreatic stellate cells.