In vitro visualization of betaine aldehyde synthesis and oxidation using hyperpolarized magnetic resonance spectroscopy

Hyla Allouche-Arnon; Ayelet Gamliel; Jacob Sosna; J Moshe Gomori; Rachel Katz-Brull

doi:10.1039/c3cc42542h

In vitro visualization of betaine aldehyde synthesis and oxidation using hyperpolarized magnetic resonance spectroscopy

Chem Commun (Camb). 2013 Aug 14;49(63):7076-8. doi: 10.1039/c3cc42542h.

Authors

Hyla Allouche-Arnon¹, Ayelet Gamliel, Jacob Sosna, J Moshe Gomori, Rachel Katz-Brull

Affiliation

¹ Department of Radiology, Hadassah-Hebrew University Medical Center, Jerusalem, 91120, Israel.

PMID: 23823928
DOI: 10.1039/c3cc42542h

Abstract

Real-time monitoring of betaine aldehyde metabolism at high temporal resolution was accomplished using a hyperpolarized choline analog and (13)C-NMR. This represents the first observation of an aldehyde intermediate on hyperpolarized MR and opens the way for kinetic studies of oxidase/dehydrogenase enzymes in vitro and in vivo.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alcohol Oxidoreductases / metabolism
Betaine / analogs & derivatives*
Betaine / chemistry
Carbon Isotopes / chemistry
Choline / chemistry
Kinetics
Magnetic Resonance Spectroscopy*
Oxidation-Reduction

Substances

Carbon Isotopes
Betaine
betaine aldehyde
Alcohol Oxidoreductases
Choline