Narrow terahertz attenuation signatures in Bacillus thuringiensis

J Biophotonics. 2014 Oct;7(10):818-24. doi: 10.1002/jbio.201300042. Epub 2013 Jul 2.

Abstract

Terahertz absorption signatures from culture-cultivated Bacillus thuringiensis were measured with a THz photomixing spectrometer operating from 400 to 1200 GHz. We observe two distinct signatures centered at ∼955 and 1015 GHz, and attribute them to the optically coupled particle vibrational resonance (surface phonon-polariton) of Bacillus spores. This demonstrates the potential of the THz attenuation signatures as "fingerprints" for label-free biomolecular detection.

Keywords: Bacillus thuringiensis; Terahertz; spectroscopy; spore; surface mode; vegetative cells.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Bacillus thuringiensis / chemistry*
  • Bacillus thuringiensis / ultrastructure
  • Microscopy, Electron, Scanning
  • Spores, Bacterial / chemistry*
  • Spores, Bacterial / ultrastructure
  • Terahertz Spectroscopy / instrumentation
  • Terahertz Spectroscopy / methods*
  • Vibration