Abstract
Reactive oxygen and nitrogen species are both regulators and effectors of microglial activation, and assays of these oxidants can be used as a measure of acute and chronic activation of microglial cells. Here we describe quick methods to assess the production of superoxide, hydrogen peroxide, nitric oxide, and peroxynitrite by microglia.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Humans
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Microglia / metabolism*
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Nitric Oxide / metabolism
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Peroxynitrous Acid
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Reactive Nitrogen Species / metabolism*
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Reactive Oxygen Species / metabolism*
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Superoxides / metabolism
Substances
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Reactive Nitrogen Species
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Reactive Oxygen Species
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Superoxides
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Peroxynitrous Acid
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Nitric Oxide