Analysis of microglial production of reactive oxygen and nitrogen species

Methods Mol Biol. 2013:1041:103-11. doi: 10.1007/978-1-62703-520-0_12.

Abstract

Reactive oxygen and nitrogen species are both regulators and effectors of microglial activation, and assays of these oxidants can be used as a measure of acute and chronic activation of microglial cells. Here we describe quick methods to assess the production of superoxide, hydrogen peroxide, nitric oxide, and peroxynitrite by microglia.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Humans
  • Microglia / metabolism*
  • Nitric Oxide / metabolism
  • Peroxynitrous Acid
  • Reactive Nitrogen Species / metabolism*
  • Reactive Oxygen Species / metabolism*
  • Superoxides / metabolism

Substances

  • Reactive Nitrogen Species
  • Reactive Oxygen Species
  • Superoxides
  • Peroxynitrous Acid
  • Nitric Oxide