Structural foundations for the O2 resistance of Desulfomicrobium baculatum [NiFeSe]-hydrogenase

Chem Commun (Camb). 2013 Aug 14;49(63):7061-3. doi: 10.1039/c3cc43619e.

Abstract

This study shows how the NiFeSe site of an anaerobically purified O2-resistant hydrogenase reacts with air to give a seleninate as the first product. Less oxidized states of the active site are readily reduced in the presence of X-rays. Reductive enzyme activation requires an efficient pathway for water escape.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Catalytic Domain
  • Crystallography, X-Ray
  • Desulfovibrio / enzymology*
  • Hydrogen / chemistry
  • Hydrogenase / chemistry
  • Hydrogenase / metabolism*
  • Oxidation-Reduction
  • Oxygen / chemistry*
  • Selenium / chemistry
  • Sulfur / chemistry

Substances

  • Sulfur
  • Hydrogen
  • nickel-iron-selenium hydrogenase
  • Hydrogenase
  • Selenium
  • Oxygen