Abstract
This study shows how the NiFeSe site of an anaerobically purified O2-resistant hydrogenase reacts with air to give a seleninate as the first product. Less oxidized states of the active site are readily reduced in the presence of X-rays. Reductive enzyme activation requires an efficient pathway for water escape.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Catalytic Domain
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Crystallography, X-Ray
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Desulfovibrio / enzymology*
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Hydrogen / chemistry
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Hydrogenase / chemistry
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Hydrogenase / metabolism*
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Oxidation-Reduction
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Oxygen / chemistry*
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Selenium / chemistry
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Sulfur / chemistry
Substances
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Sulfur
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Hydrogen
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nickel-iron-selenium hydrogenase
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Hydrogenase
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Selenium
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Oxygen