Optimization of Campylobacter growth conditions for further identification by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)

Delphine Martiny; Alain Visscher; Boudewijn Catry; Sonia Chatellier; Olivier Vandenberg

doi:10.1016/j.mimet.2013.06.018

Optimization of Campylobacter growth conditions for further identification by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)

J Microbiol Methods. 2013 Sep;94(3):221-3. doi: 10.1016/j.mimet.2013.06.018. Epub 2013 Jun 26.

Authors

Delphine Martiny¹, Alain Visscher, Boudewijn Catry, Sonia Chatellier, Olivier Vandenberg

Affiliation

¹ Department of Microbiology, Saint-Pierre University Hospital & Jules Bordet Institute, Brussels, Belgium. delphine_martiny@stpierre-bru.be

PMID: 23811211
DOI: 10.1016/j.mimet.2013.06.018

Abstract

Growth conditions - including growth medium and incubation temperature - may influence the identification of Campylobacter by MALDI-TOF MS. For each bacterial species, medical microbiologists should be aware of such potential influences on spectral data before analyzing and interpreting MALDI-TOF MS results.

Keywords: Campylobacter; Growth conditions; MALDI-TOF MS; Optimization.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Typing Techniques / methods*
Campylobacter* / chemistry
Campylobacter* / growth & development
Campylobacter* / isolation & purification
Culture Media
Odds Ratio
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
Temperature

Substances

Culture Media