Abstract
Regulation of transcriptional processes is a critical mechanism that enables efficient coordination of the synthesis of required proteins in response to environmental and cellular changes. Transcription factors require accurate activity regulation because they play a critical role as key mediators assuring specific expression of target genes. In this work, we show that cullin3-based E3 ligases have the potential to interact with a broad range of ethylene response factor (ERF)/APETALA2 (AP2) transcription factors, mediated by Math-BTB/POZ (for Meprin and TRAF [tumor necrosis factor receptor associated factor] homolog)-Broad complex, Tramtrack, Bric-a-brac/Pox virus and Zinc finger) proteins. The assembly with an E3 ligase causes degradation of their substrates via the 26S proteasome, as demonstrated for the wrinkled1 ERF/AP2 protein. Furthermore, loss of Math-BTB/POZ proteins widely affects plant development and causes altered fatty acid contents in mutant seeds. Overall, this work demonstrates a link between fatty acid metabolism and E3 ligase activities in plants and establishes CUL3-based E3 ligases as key regulators in transcriptional processes that involve ERF/AP2 family members.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Amino Acid Sequence
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Arabidopsis / genetics
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Arabidopsis / metabolism
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Arabidopsis Proteins / genetics
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Arabidopsis Proteins / metabolism*
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Carrier Proteins / genetics
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Carrier Proteins / metabolism*
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Chromatin Immunoprecipitation
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Cullin Proteins
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Cycloheximide / pharmacology
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Fatty Acids / metabolism*
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Gene Expression Regulation, Plant / drug effects
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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Microscopy, Confocal
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Molecular Sequence Data
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Nucleotidyltransferases / genetics
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Nucleotidyltransferases / metabolism
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Plants, Genetically Modified
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Proteasome Endopeptidase Complex / metabolism
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Protein Binding
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Reverse Transcriptase Polymerase Chain Reaction
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Seeds / genetics
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Seeds / metabolism
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Sequence Homology, Amino Acid
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Transcription Factors / genetics
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Transcription Factors / metabolism
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Two-Hybrid System Techniques
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Ubiquitin-Protein Ligases / genetics
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Ubiquitin-Protein Ligases / metabolism*
Substances
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AT1G26830 protein, Arabidopsis
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Arabidopsis Proteins
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BPM1 protein, Arabidopsis
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Carrier Proteins
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Cullin Proteins
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Fatty Acids
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Transcription Factors
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WRINKLED1 protein, Arabidopsis
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cullin 3B protein, Arabidopsis
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Green Fluorescent Proteins
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Cycloheximide
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Ubiquitin-Protein Ligases
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Nucleotidyltransferases
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REV1 protein, Arabidopsis
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Proteasome Endopeptidase Complex
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ATP dependent 26S protease