Abstract
Rapid one-step modification of thrombomodulin with alkylamine derivatives such as azide, biotin, and PEG is achieved using an evolved sortase (eSrtA) mutant. The feasibility of a point-of-care scheme is demonstrated herein to site-specifically immobilize azido-thrombomodulin on sterilized commercial ePTFE vascular grafts, which exhibit superior thromboresistance compared with commercial heparin-coated grafts in a primate model of acute graft thrombosis.
Keywords:
antifouling; biocompatible materials; biomedical applications; thrombomodulin; thrombosis.
Copyright © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Amines / chemistry*
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Amines / metabolism
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Aminoacyltransferases / metabolism
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Animals
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Azides / chemistry
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Azides / metabolism
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Bacterial Proteins / metabolism
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Biotin / chemistry
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Biotin / metabolism
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Blood Platelets / chemistry
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Blood Platelets / metabolism
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Coated Materials, Biocompatible / chemistry
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Coated Materials, Biocompatible / metabolism
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Cysteine Endopeptidases / metabolism
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Disease Models, Animal
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Heparin / chemistry
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Heparin / metabolism
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Heparin / therapeutic use
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Immobilized Proteins / chemistry
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Immobilized Proteins / metabolism
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Papio
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Polyethylene Glycols / chemistry
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Polyethylene Glycols / metabolism
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Polytetrafluoroethylene / chemistry
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Polytetrafluoroethylene / metabolism
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Staphylococcus aureus / enzymology
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Thrombomodulin / chemistry*
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Thrombomodulin / metabolism
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Thrombosis / drug therapy
Substances
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Amines
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Azides
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Bacterial Proteins
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Coated Materials, Biocompatible
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Immobilized Proteins
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Thrombomodulin
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Polyethylene Glycols
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Biotin
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Polytetrafluoroethylene
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Heparin
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Aminoacyltransferases
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sortase A
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Cysteine Endopeptidases