Abstract
With the use of a chemistry-based artificial restriction DNA cutter (combination of Ce(IV)-EDTA and a pair of pcPNA), both an antibiotic-resistance gene and a fluorescent reporter protein gene were incorporated into the targeted site through homologous recombination in human cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Line
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Cerium / chemistry
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Cerium / metabolism
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DNA / administration & dosage*
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DNA / genetics
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DNA / metabolism
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Drug Resistance, Microbial
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Edetic Acid / chemistry
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Edetic Acid / metabolism
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Electroporation
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Homologous Recombination*
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Humans
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Mutagenesis, Insertional*
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Peptide Nucleic Acids / chemistry
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Peptide Nucleic Acids / metabolism
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Proteins / genetics
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Transfection / methods*
Substances
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Peptide Nucleic Acids
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Proteins
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Cerium
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DNA
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Edetic Acid