An artificial restriction DNA cutter for site-selective gene insertion in human cells

Chem Commun (Camb). 2013 Aug 4;49(60):6764-6. doi: 10.1039/c3cc43261k.

Abstract

With the use of a chemistry-based artificial restriction DNA cutter (combination of Ce(IV)-EDTA and a pair of pcPNA), both an antibiotic-resistance gene and a fluorescent reporter protein gene were incorporated into the targeted site through homologous recombination in human cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • Cerium / chemistry
  • Cerium / metabolism
  • DNA / administration & dosage*
  • DNA / genetics
  • DNA / metabolism
  • Drug Resistance, Microbial
  • Edetic Acid / chemistry
  • Edetic Acid / metabolism
  • Electroporation
  • Homologous Recombination*
  • Humans
  • Mutagenesis, Insertional*
  • Peptide Nucleic Acids / chemistry
  • Peptide Nucleic Acids / metabolism
  • Proteins / genetics
  • Transfection / methods*

Substances

  • Peptide Nucleic Acids
  • Proteins
  • Cerium
  • DNA
  • Edetic Acid