Neutral liposomes containing crown ether-lipids as potential DNA vectors

Guido Angelini; Michela Pisani; Giovanna Mobbili; Milvia Marini; Carla Gasbarri

doi:10.1016/j.bbamem.2013.06.003

Neutral liposomes containing crown ether-lipids as potential DNA vectors

Biochim Biophys Acta. 2013 Nov;1828(11):2506-12. doi: 10.1016/j.bbamem.2013.06.003. Epub 2013 Jun 14.

Authors

Guido Angelini¹, Michela Pisani, Giovanna Mobbili, Milvia Marini, Carla Gasbarri

Affiliation

¹ Dipartimento di Farmacia, Università "G. d'Annunzio" di Chieti-Pescara, via dei Vestini, 66100 Chieti, Italy.

PMID: 23774523
DOI: 10.1016/j.bbamem.2013.06.003

Abstract

Three crown ether derivatives, 1,2-O-dioleoyl-3-O-{2-[(12-crown-4)methoxy]ethyl}-sn-glycerol (12C4L), 1,2-O-dioleoyl-3-O-{2-[(15-crown-5)methoxy]ethyl}-sn-glycerol (15C5L) and 2,3-naphtho-15-crown-5 (NAP5), have been incorporated into 1-palmitoyl-2-oleoyl-phosphatydilcholine (POPC) liposomes. The size of the crown ether and the lipophilic moiety of 12C4L, 15C5L and NAP5 influence the stability and the properties of the extruded POPC liposomes determined at 25°C in buffered aqueous solution at pH7.4. The investigated liposomes are zwitterionic for POPC headgroups but can be turned into cationic aggregates in the presence of divalent cations. The capability of these systems to complex DNA has been demonstrated by SAXS experiments.

Keywords: Crown ether-lipid; DNA complex; Kinetics; Liposome; Turbidity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Crown Ethers / chemistry*
DNA / chemistry*
Lipids / chemistry*
Liposomes*
Scattering, Small Angle

Substances

Crown Ethers
Lipids
Liposomes
DNA