Abstract
Antigen-specific γδ T cells represent an early innate defense known to play an important role in anti-mycobacterial immunity. We have investigated the immune functions of Vγ9Vδ2 T cells from Broncho-Alveolar lavages (BAC) samples of active TB patients. We observed that BAC Vγ9Vδ2 T cells presented a strong down-modulation of CD3 expression compared with Vγ9Vδ2 T cells from peripheral blood. Furthermore, Vγ9Vδ2 T cells mainly showed a central memory phenotype, expressed high levels of NK inhibitory receptors and TEMRA cells showed low expression of CD16 compared to circulating Vγ9Vδ2 T cells. Interestingly, the ability of BAC Vγ9Vδ2 T cells to respond to antigen stimulation was dramatically reduced, differently from blood counterpart. These observations indicate that γδ T cell functions are specifically impaired in situ by active TB, suggesting that the alveolar ambient during tuberculosis may affect resident γδ T cells in comparison to circulating cells.
Keywords:
Lung; Mycobacterium; Primary infection; γδ T cells.
Copyright © 2013 Elsevier Inc. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adolescent
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Adult
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Bronchoalveolar Lavage Fluid / immunology
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CD3 Complex / immunology
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CD3 Complex / metabolism
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Female
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Flow Cytometry
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Humans
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Immunologic Memory / immunology
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Immunophenotyping
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Interferon-gamma / immunology
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Interferon-gamma / metabolism
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Leukocytes, Mononuclear / immunology
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Leukocytes, Mononuclear / metabolism
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Lung / immunology*
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Lung / metabolism
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Lung / pathology
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Male
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Middle Aged
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Receptors, Antigen, T-Cell, gamma-delta / immunology*
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Receptors, Antigen, T-Cell, gamma-delta / metabolism
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Receptors, IgG / immunology
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Receptors, IgG / metabolism
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Receptors, Natural Killer Cell / immunology
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Receptors, Natural Killer Cell / metabolism
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T-Lymphocytes / immunology*
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T-Lymphocytes / metabolism
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Tuberculosis / blood
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Tuberculosis / immunology*
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Tuberculosis / metabolism
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Tumor Necrosis Factor-alpha / immunology
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Tumor Necrosis Factor-alpha / metabolism
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Young Adult
Substances
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CD3 Complex
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Receptors, Antigen, T-Cell, gamma-delta
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Receptors, IgG
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Receptors, Natural Killer Cell
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Tumor Necrosis Factor-alpha
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Interferon-gamma