Abstract
Two new lanostanoids, 3α-acetoxy-22-oxo-5α-lanosta-8,24-dien-21-oic acid, named tsugaric acid D (1) and 16α-hydroxy-3-oxo-5α-lanosta-6,8,24(24(1))-trien-21-oic acid, named tsugaric acid E (2) were isolated from the fruit bodies of Ganoderma tsugae. The structures 1 and 2 were determined by spectroscopic methods. Compound 1 and known compounds 3 and 6 exhibited significant inhibitory effects on xanthine oxidase (XO) activity with an IC50 values of 90.2±24.2, 116.1±3.0, and 181.9±5.8 μM, respectively. Known compound 5 was able to protect human keratinocytes against damage induced by UVB light, which showed 5 could protect keratinocytes from photodamage. The 1 and 5 μM 1 combined with 5 μM cisplatin, respectively, enhanced the cytotoxicity induced by cisplatin. It suggested that 1 and 5 μM 1 combined with low dose of cisplatin may enhance the therapeutic efficacy of cisplatin and reduce side effect and cisplatin resistant.
Keywords:
Antioxidant; Cytotoxicity; Ganoderma tsugae; Lanostanoids; Photodamage.
Copyright © 2013 Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antineoplastic Agents, Phytogenic / chemistry
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Antineoplastic Agents, Phytogenic / isolation & purification
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Antineoplastic Agents, Phytogenic / pharmacology
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Antineoplastic Agents, Phytogenic / therapeutic use
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Antioxidants / chemistry
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Antioxidants / isolation & purification
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Antioxidants / pharmacology
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Antioxidants / therapeutic use*
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Biological Products / chemistry
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Biological Products / pharmacology
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Biological Products / therapeutic use*
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Cisplatin / pharmacology
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Cisplatin / therapeutic use
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Fruiting Bodies, Fungal / chemistry
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Ganoderma / chemistry*
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Herb-Drug Interactions
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Humans
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Inhibitory Concentration 50
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Keratinocytes / drug effects*
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Keratinocytes / radiation effects
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Lanosterol / analogs & derivatives*
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Lanosterol / chemistry
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Lanosterol / isolation & purification
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Lanosterol / pharmacology
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Lanosterol / therapeutic use
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Male
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Molecular Structure
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Phytotherapy
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Prostatic Neoplasms / drug therapy*
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Radiation Injuries, Experimental / drug therapy
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Skin Diseases / prevention & control
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Xanthine Oxidase / antagonists & inhibitors*
Substances
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Antineoplastic Agents, Phytogenic
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Antioxidants
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Biological Products
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tsugaric acid
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Lanosterol
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Xanthine Oxidase
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Cisplatin