Insect antennae are among the most sensitive and selective chemical-sensing organs in the animal kingdom. Insects can perceive picograms of specific volatile organic compounds per cubic meter of air in milliseconds, which is far below the detection thresholds of current analytical devices. These exceptional sensing abilities have many uses in the context of insect biotechnology. Living specimens or parts of them, such as isolated antennae or individual proteins, can serve as biosensors in the field. As volatiles occur in a crude mixture in the environment, knowing which trigger-volatiles are crucial for the insects' perception of specific incidents is of great value. This knowledge promotes the development of selective sensors for applications, such as fire detection. In this chapter, we discuss the different technical procedures for the preparation and use of insect-based biosensors for the detection of organic volatiles, including those based on insect behavior, insect olfactory proteins, and biomimetic sensing units. We also consider the use of these applications in portable devices outside the laboratory under field conditions.