Complete sequences of two plasmids in a blaNDM-1-positive Klebsiella oxytoca isolate from Taiwan

Antimicrob Agents Chemother. 2013 Aug;57(8):4072-6. doi: 10.1128/AAC.02266-12. Epub 2013 Jun 10.

Abstract

Genetic determinants of a bla(NDM-1)-positive, multidrug-resistant bacterial isolate that caused active infection was investigated by DNA sequencing. Two plasmids, pKOX_NDM1 and pKOX-R1, were identified for the Klebsiella oxytoca strain E718. Sequence annotation revealed a bla(NDM-1) gene in pKOX_NDM1 and two extended-spectrum β-lactamase producers (bla(CTX-M-3) and blaSHV-12) and a wide array of resistance genes in pKOX-R1. These findings highlight the difficulty in treating multidrug-resistant bacterial infections and the potential danger of emerging resistant enterobacteria.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aminoglycosides / pharmacology
  • Anti-Bacterial Agents / pharmacology
  • Conjugation, Genetic
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Genes, Bacterial*
  • Genes, MDR
  • Humans
  • Klebsiella Infections / diagnosis
  • Klebsiella oxytoca / drug effects
  • Klebsiella oxytoca / enzymology
  • Klebsiella oxytoca / genetics*
  • Klebsiella oxytoca / isolation & purification
  • Microbial Sensitivity Tests
  • Plasmids / genetics*
  • Taiwan
  • beta-Lactamases / genetics*

Substances

  • Aminoglycosides
  • Anti-Bacterial Agents
  • beta-lactamase CTX-M-3
  • beta-lactamase SHV-12
  • beta-Lactamases
  • beta-lactamase NDM-1