PCR-based diagnosis of prosthetic joint infection

Xinhua Qu; Zanjing Zhai; Huiwu Li; Haowei Li; Xuqiang Liu; Zhenan Zhu; You Wang; Guangwang Liu; Kerong Dai

doi:10.1128/JCM.00657-13

PCR-based diagnosis of prosthetic joint infection

J Clin Microbiol. 2013 Aug;51(8):2742-6. doi: 10.1128/JCM.00657-13. Epub 2013 Jun 5.

Authors

Xinhua Qu¹, Zanjing Zhai, Huiwu Li, Haowei Li, Xuqiang Liu, Zhenan Zhu, You Wang, Guangwang Liu, Kerong Dai

Affiliation

¹ Department of Orthopedics, Shanghai Key Laboratory of Orthopedic Implants, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.

Abstract

We performed a meta-analysis to evaluate use of PCR assays for diagnosis of prosthetic joint infection (PJI). The pooled sensitivity and specificity were 0.86 (95% confidence interval [CI], 0.77 to 0.92) and 0.91 (CI, 0.81 to 0.96), respectively. Subgroup analyses showed that use of tissue samples may improve sensitivity, and quantitative PCR and sonication of prostheses fluid may improve specificity. The results showed that PCR is reliable and accurate for detection of PJI.

Publication types

Evaluation Study
Meta-Analysis
Research Support, Non-U.S. Gov't

MeSH terms

Humans
Microbiological Techniques / methods*
Molecular Diagnostic Techniques / methods*
Osteoarthritis / diagnosis*
Polymerase Chain Reaction / methods*
Prosthesis-Related Infections / diagnosis*
Sensitivity and Specificity