Effects of salvianolic acid and notoginseng triterpenes on angiogenesis in EA-hy926 cells in vitro

Chin J Nat Med. 2013 May;11(3):254-7. doi: 10.1016/S1875-5364(13)60024-2.

Abstract

Aim: To investigate the different effects of salvianolic acid and notoginseng triterpenes on proliferation, angiogenesis and expression of vascular endothelial growth factor in EA-hy926 cells in vitro.

Methods: EA-hy926 cells were cultured in vitro. Salvianolic acid and notoginseng triterpenes at concentrations of 0.4, 0.8 and 1.2 mg·L(-1) were used to culture EA-hy926 cells. EA-hy926 cells in a blank control group were grown in culture solution only. Viability of cells was assessed by CCK-8, and after treated for 12 h, capillary-like structures were examined. After 24 h culture, the expression of VEGF was detected by real-time PCR.

Results: Salvianolic acid at 0.4, 0.8 mg·L(-1), the same as notoginseng triterpenes, increased VEGF content in EA-hy926 cells. Expression of VEGF protein in the salvianolic acid at 1.2 mg·L(-1) group, was up-regulated as compared with notoginseng triterpenes group (P < 0.05).

Conclusion: Salvianolic acid and notoginseng triterpenes can promote EA-hy926 cell proliferation, angiogenesis and expression of VEGF protein. This analysis also provided evidence that salvianolic acid had the better effects as compared with notoginseng triterpenes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkenes / pharmacology*
  • Cell Line, Tumor
  • Cell Proliferation / drug effects
  • Coronary Stenosis / drug therapy*
  • Coronary Stenosis / genetics
  • Coronary Stenosis / metabolism
  • Coronary Stenosis / physiopathology
  • Drugs, Chinese Herbal / pharmacology*
  • Endothelial Cells / drug effects*
  • Endothelial Cells / metabolism
  • Humans
  • Neovascularization, Pathologic / drug therapy
  • Neovascularization, Pathologic / genetics
  • Neovascularization, Pathologic / metabolism
  • Neovascularization, Pathologic / physiopathology
  • Panax notoginseng / chemistry*
  • Polyphenols / pharmacology*
  • Triterpenes / pharmacology*
  • Vascular Endothelial Growth Factor A / genetics
  • Vascular Endothelial Growth Factor A / metabolism

Substances

  • Alkenes
  • Drugs, Chinese Herbal
  • Polyphenols
  • Triterpenes
  • Vascular Endothelial Growth Factor A
  • salvianolic acid