Improved Mannanase Production from Penicillium occitanis by Fed-Batch Fermentation Using Acacia Seeds

Monia Blibech; Raoudha Ellouz Ghorbel; Fatma Chaari; Ilyes Dammak; Fatma Bhiri; Mohamed Neifar; Semia Ellouz Chaabouni

doi:10.5402/2011/938347

Improved Mannanase Production from Penicillium occitanis by Fed-Batch Fermentation Using Acacia Seeds

ISRN Microbiol. 2011 Oct 24:2011:938347. doi: 10.5402/2011/938347. Print 2011.

Authors

Monia Blibech¹, Raoudha Ellouz Ghorbel, Fatma Chaari, Ilyes Dammak, Fatma Bhiri, Mohamed Neifar, Semia Ellouz Chaabouni

Affiliation

¹ Unité Enzymes et Bioconversion, Ecole National d'ingénieurs de Sfax, Université de Sfax, Route de Soukra 3038, Sfax, Tunisia.

Abstract

By applying a fed-batch strategy, production of Penicillium occitanis mannanases could be almost doubled as compared to a batch cultivation on acacia seeds (76 versus 41 U/mL). Also, a 10-fold increase of enzyme activities was observed from shake flask fermentation to the fed-batch fermentation. These production levels were 3-fold higher than those obtained on coconut meal. The high mannanase production using acacia seeds powder as inducer substrate showed the suitability of this culture process for industrial-scale development.