Rapid discrimination of Acinetobacter baumannii international clone II lineage by pyrosequencing SNP analyses of bla(OXA-51-like) genes

Mari Matsui; Satowa Suzuki; Masato Suzuki; Yoshichika Arakawa; Keigo Shibayama

doi:10.1016/j.mimet.2013.05.014

Rapid discrimination of Acinetobacter baumannii international clone II lineage by pyrosequencing SNP analyses of bla(OXA-51-like) genes

J Microbiol Methods. 2013 Aug;94(2):121-124. doi: 10.1016/j.mimet.2013.05.014. Epub 2013 May 26.

Authors

Mari Matsui¹, Satowa Suzuki², Masato Suzuki², Yoshichika Arakawa³, Keigo Shibayama²

Affiliations

¹ Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo, Japan. Electronic address: m-matsui@nih.go.jp.
² Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo, Japan.
³ Department of Bacteriology, Nagoya University Graduate School of Medicine, Nagoya Japan.

PMID: 23719027
DOI: 10.1016/j.mimet.2013.05.014

Abstract

We found that Acinetobacter baumannii international clone II generally possesses unique GTA sequence at nucleotide positions 106-108 in the bla(OXA-51-like) genes. We exploited this to develop an easy and rapid method for discrimination of international clone II from other A. baumannii by employing pyrosequencing analyses of single nucleotide polymorphisms.

Keywords: Acinetobacter baumannii; Pyrosequence; bla(OXA-51-like).

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Acinetobacter baumannii / classification
Acinetobacter baumannii / enzymology
Acinetobacter baumannii / genetics*
Acinetobacter baumannii / isolation & purification
Bacterial Typing Techniques / methods*
Base Sequence
High-Throughput Nucleotide Sequencing / methods*
Molecular Sequence Data
Phylogeny
Polymorphism, Single Nucleotide*
beta-Lactamases / genetics

Substances

beta-Lactamases
beta-lactamase OXA-51, Acinetobacter baumannii