Objective: To study the absorption characteristics of four components from Naoxintong capsule in intestines.
Method: In vitro everted gut sac method was adopted for preparing the intestinal absorption solution of Naoxintong capsule. UPLC was used to detect the content of chemical components in different intestinal segments, and comparing the results with the absorption of chemical components of Naoxintong capsule in each intestinal segment. The time-accumulative absorption curve was drawn to observe the changes in the accumulative absorption concentration with time.
Result: Four ingredients of Naoxintong capsule can be detected in intestinal absorption solution, they are ferulic acid, paeoniflorin, salvianolic acid B and hydroxysafflor yellow A. Specifically, the accumulative absorption concentrations of ferulic acid, salvianolic acid B and paeoniflorin in ileum and rear jejunum segments were higher than that in front and middle jejunum segments; the absorption of ferulic acid, paeoniflorin, salvianolic acid B and hydroxysafflor yellow A did not reach saturated conditions in 3 hours.
Conclusion: Ferulic acid, paeoniflorin, salvianolic acid B and hydroxysafflor yellow A are absorbed in the whole intestine. Ferulic acid, paeoniflorin and salvianolic acid B may be absorbed in specific segments.