miR-506 regulates epithelial mesenchymal transition in breast cancer cell lines

PLoS One. 2013 May 22;8(5):e64273. doi: 10.1371/journal.pone.0064273. Print 2013.

Abstract

Epithelial-mesenchymal transition (EMT) is an important parameter related to breast cancer survival. Among several microRNAs predicted to target EMT-related genes, miR-506 is a novel miRNA found to be significantly related to breast cancer patient survival in a meta-analysis. miR-506 suppressed the expression of mesenchymal genes such as Vimentin, Snai2, and CD151 in MDA-MB-231 human breast cancer cell line. Moreover, NF-κB bound to the upstream promoter region of miR-506 to suppress transcription. Overexpression of miR-506 inhibited TGFβ-induced EMT and suppressed adhesion, invasion, and migration of MDA-MB-231 cells. From these results, we concluded that miR-506 plays a key role in the process of EMT through posttranslational control of EMT-related genes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Breast Neoplasms / pathology*
  • Cell Adhesion / physiology
  • Cell Line, Tumor
  • Cell Movement / physiology
  • Epithelial-Mesenchymal Transition / physiology*
  • Female
  • Humans
  • MicroRNAs / physiology*
  • NF-kappa B / metabolism
  • Neoplasm Invasiveness
  • Promoter Regions, Genetic

Substances

  • MIRN506 microRNA, human
  • MicroRNAs
  • NF-kappa B

Grants and funding

This study was supported by the National Research Foundation of Korea, funded by the Ministry of Education, Science and Technology (2012M2B2A9A02029603 and 2012M3A9B2029132 to W.-Y.P.). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript